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Culture and molecular methods compared for actinobacteria on water-damaged building material
Molecular analysis of mold-colonized water-damaged wall material identified more actinobacterial genera than culture-based methods on the same samples.
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This library reports what studies found. An entry describes a study's design, its findings in its own terms, and its limits. Nothing here is a claim about what a Sage Co. home does, delivers, or prevents.
Last reviewed August 30, 2026.
What they studied
- Sampling of mold-colonized wall materials from water-damaged buildings.
- Parallel characterization by cultivation and by 16S rRNA gene molecular analysis.
- Comparison of the genera recovered by each method.
What they found
- The authors reported 47 actinobacterial genera detected by molecular analysis versus 31 by cultivation.
- Mold-colonized materials were reported to be co-colonized by actinobacteria in the sampled set.
- The authors reported that culture-based sampling underestimated the bacterial diversity present.
What this study does not establish
- A methods comparison on a limited sample set; it does not measure airborne exposure.
- Detection of genetic material does not establish viability, release, or inhalation.
- No health outcomes were measured.
- This entry reports the study. It is not a statement about any building Sage Co. designs or constructs.
Source
Schäfer J, Jäckel U, Kämpfer P (2010). Analysis of Actinobacteria from mold-colonized water damaged building material. Systematic and Applied Microbiology 33(5):260–268.
actinomycetessampling methodsbuilding materials
Elsewhere under Moisture & Mold
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- Actinomycetes and non-tuberculous mycobacteria measured against respiratory findings in a water-damaged office building
- Review of actinobacteria in indoor environments and reported respiratory effects
- Inflammatory response in mice after instillation of Streptomyces spores from a moldy building
- Co-occurrence of bacterial and fungal secondary metabolites measured in moisture-damaged homes
- Chaetomium globosum prevalence in surveyed water-damaged buildings and metabolite production on building materials
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- Associations between fungal species and specific water-damaged building materials
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- Immune response measured after single versus repeated Stachybotrys lung exposures in mice
- Nasal and olfactory effects of satratoxin G measured in rhesus monkeys
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- Lung inflammation measured in mice exposed to Aspergillus versicolor spores from a moisture-damaged building
- Inflammatory gene expression measured in macrophages exposed to toxins from damp-building fungi
- Lung responses measured in mice exposed to purified toxins from building-isolated Penicillium species
- Human brain cell responses measured at satratoxin concentrations reported from water-damaged buildings
- Time-series and clinical-trial data reported on illness in water-damaged building occupants
- Review of proposed injury mechanisms from exposure to water-damaged buildings
- Neuropsychological and electrocortical measures compared between mold-exposed subjects and controls
